Details-enriched characterization of cell populations. Morphological details from photographs can incorporate towards the richness of flow cytometry data. For example, the figure demonstrates an or else common apoptosis assay making use of Annexin V and PI, incorporating cell imaging to characterize cells in each populace to reveal morphologically unique features. These insights couldn't are already acquired from multiplex staining by itself.
As samples are acquired about the Attune CytPix Flow Cytometer, the high-velocity brightfield digital camera captures and shops photographs of detected gatherings at a level up to 6,000 illustrations or photos per next, depending upon the flow charge and graphic dimension. For bigger overall flexibility, Attune Cytometric Application lets you change the image seize frequency as wanted and choose a certain gate for impression capture, if essential.
Combine acoustic focusing technology with substantial-parameter Investigation for adaptable spectral unmixing and traditional workflows.
Find out new insights into your experimental details by visualizing populations using combined fluorescence and morphology facts. Our computer software interface wizards allow downsampling, concatenation (merged data files) and dimensionality resources that are developed into the software for an intuitive, streamlined details Examination expertise.
"A picture is not pretty worthy of one thousand dots, but it truly helps you to see photos. It is great to view photographs that correlate with staining. For example, AnnexinV-positive cells which can be morphologically distinctive than nutritious cells, or CD14+ monocytes which might be bigger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it is illuminating to discover the number of doublets slip via standard singlet gates, and terrific to validate the status of unexpected double-positive activities in my Assessment."
Pair your Attune flow cytometer by having an autosampler for increased workflow performance, and include robotic integration for max throughput.
Our experts will structure panels to suit your needs as often times you need—totally free, no products obtain essential.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can depend on for consistent and trusted performance. The Attune CytPix product incorporates a significant-speed brightfield digicam and AI-driven image Assessment software program, with current upgrades like machine Studying equipment and an intuitive user interface, enabling Increased facts precision, accuracy, and more rapidly insights into sample biology.
Perhaps most of all, these events include cells of Evidently different phenotypes which may lead to incorrect conclusions concerning double optimistic functions (especially in scarce populations).
AI-pushed automatic impression Examination Together with the Attune Cytometric Application translates event characteristics into unique morphology parameters which can be coupled with typical fluorescence and scatter parameters to expose hugely reproducible populace variations and exceptional subsets.
We previously shown the power of imaging Car or truck-T/Ramos cell interactions. Let’s look at just the population of finest fascination, the double optimistic occasions, to learn more. We are able to now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to further analyze the options of these populations and refine gating on these situations, expanding info robustness.
Crafted-in dimensionality reduction equipment more info improved resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to confirm predicted population resolution in lysed entire human blood. Consumer can see occasions from Just about every cluster for Visible confirmation of cell phenotypes.
No wash/no lyse leukocyte Assessment with imaging. Cells were being obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Specialist UX/UI enables consumers to train AI products for expanded purposes. (A) The “Educate Model” feature presents annotation equipment and authentic-time design teaching precision.
Gating on these extended parameters helps you to promptly and precisely determine populations of fascination and make sure gating method with little or no handbook review. The Attune software program gives use of state-of-the-artwork device Understanding Investigation applications within an expertly created person interface, which makes it exceptionally user friendly.
Since the cell line experienced Beforehand been analyzed over the Attune CytPix Flow Cytometer, the scientists went back again to the images and were being able to document the microbial an infection no less than 5 times previously.
Agilent Price Guarantee ensures you at the least a decade of instrument use within the day you buy. Normally, We are going to credit history you Along with the residual price of the program toward an upgraded product.
Below, we show that by gating procedures making use of picture-centered quantitative parameters can distinguish interacting cells from coincident activities to far more properly review interacting cells.